Studies of pregnancy-associated murine serum proteins

A study of the Electrophoretic Pattern of the serum Proteins

Identification of proteins associated with murine cytomegalovirus virions.

Proteins associated with the murine cytomegalovirus (MCMV) viral particle were identified by a combined approach of proteomic and genomic methods. Purified MCMV virions were dissociated by complete denaturation and subjected to either separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel digestion or treated directly by in-solution tryptic digestion. Peptides were s...

Identification of proteins associated with murine gammaherpesvirus 68 virions.

Murine gammaherpesvirus 68 (MHV68 [also known as gammaHV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying gammaherpesvirus virion composition. Extracellular MHV68 virions were isolated, and abundant virion-associated proteins were identified by mass spectrometry. Five nucleocapsid protein homologues, the tegument p...

Electrophoretic studies of the serum proteins in virus hepatitis.

The abnormal electrophoretic patterns of the serum proteins in the acute stage of virus hepatitis were first described by Gray and Barron in 1943 (1) and have since been confirmed by several investigators (2-5). In the last few years there has been considerable interest (6-13) in cases of hepatitis progressing to chronic liver disease, or developing post-necrotic cirrhosis. Except for the study...

Electrophoretic studies of the serum proteins in biliary cirrhosis.

Electrophoretic runs were performed with standard apparatus and technique, the experimental details having been reported previously (4). The buffer was sodium diethylbarbiturate (veronal) of pH 8.6 and ionic strength 0.1. In the estimation of the fractions as per cent of the serum proteins both ascending and descending patterns were used, the areas being divided by ordinates from the nadirs bet...